Ontology highlight
ABSTRACT:
SUBMITTER: Jiang B
PROVIDER: S-EPMC4828452 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
Jiang Baojie B Zhang Ruiqin R Feng Dan D Wang Fangzhong F Liu Kuimei K Jiang Yi Y Niu Kangle K Yuan Quanquan Q Wang Mingyu M Wang Hailong H Zhang Youming Y Fang Xu X
Frontiers in microbiology 20160412
The lack of selective markers has been a key problem preventing multistep genetic engineering in filamentous fungi, particularly for industrial species such as the lignocellulose degrading Penicillium oxalicum JUA10-1(formerly named as Penicillium decumbens). To resolve this problem, we constructed a genetic manipulation system taking advantage of two established genetic systems: the Cre-loxP system and Tet-on system in P. oxalicum JUA10-1. This system is efficient and convenient. The expression ...[more]