Ontology highlight
ABSTRACT:
SUBMITTER: Chen YT
PROVIDER: S-EPMC525470 | biostudies-literature | 2004 Nov
REPOSITORIES: biostudies-literature
Chen You-Tzung YT Liu Pentao P Bradley Allan A
Molecular and cellular biology 20041101 22
Gene trapping in mouse embryonic stem cells is an important genetic approach that allows simultaneous mutation of genes and generation of corresponding mutant mice. We designed a selection scheme with drug selection markers and Cre/loxP technology which allows screening of gene trap events that responded to a signaling molecule in a 96-well format. Nine hundred twenty gene trap clones were assayed, and 258 were classified as gene traps induced by in vitro differentiation. Sixty-five of the in vi ...[more]