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Micron-scale plasma membrane curvature is recognized by the septin cytoskeleton.


ABSTRACT: Cells change shape in response to diverse environmental and developmental conditions, creating topologies with micron-scale features. Although individual proteins can sense nanometer-scale membrane curvature, it is unclear if a cell could also use nanometer-scale components to sense micron-scale contours, such as the cytokinetic furrow and base of neuronal branches. Septins are filament-forming proteins that serve as signaling platforms and are frequently associated with areas of the plasma membrane where there is micron-scale curvature, including the cytokinetic furrow and the base of cell protrusions. We report here that fungal and human septins are able to distinguish between different degrees of micron-scale curvature in cells. By preparing supported lipid bilayers on beads of different curvature, we reconstitute and measure the intrinsic septin curvature preference. We conclude that micron-scale curvature recognition is a fundamental property of the septin cytoskeleton that provides the cell with a mechanism to know its local shape.

SUBMITTER: Bridges AA 

PROVIDER: S-EPMC4828694 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature

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Micron-scale plasma membrane curvature is recognized by the septin cytoskeleton.

Bridges Andrew A AA   Jentzsch Maximilian S MS   Oakes Patrick W PW   Occhipinti Patricia P   Gladfelter Amy S AS  

The Journal of cell biology 20160404 1


Cells change shape in response to diverse environmental and developmental conditions, creating topologies with micron-scale features. Although individual proteins can sense nanometer-scale membrane curvature, it is unclear if a cell could also use nanometer-scale components to sense micron-scale contours, such as the cytokinetic furrow and base of neuronal branches. Septins are filament-forming proteins that serve as signaling platforms and are frequently associated with areas of the plasma memb  ...[more]

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