Ontology highlight
ABSTRACT:
SUBMITTER: Duellberg C
PROVIDER: S-EPMC4829430 | biostudies-literature | 2016 Apr
REPOSITORIES: biostudies-literature
Duellberg Christian C Cade Nicholas I NI Holmes David D Surrey Thomas T
eLife 20160406
The function of microtubules relies on their ability to switch between phases of growth and shrinkage. A nucleotide-dependent stabilising cap at microtubule ends is thought to be lost before this switch can occur; however, the nature and size of this protective cap are unknown. Using a microfluidics-assisted multi-colour TIRF microscopy assay with close-to-nm and sub-second precision, we measured the sizes of the stabilizing cap of individual microtubules. We find that the protective caps are fo ...[more]