Unknown

Dataset Information

0

Serotonin modulates insect hemocyte phagocytosis via two different serotonin receptors.


ABSTRACT: Serotonin (5-HT) modulates both neural and immune responses in vertebrates, but its role in insect immunity remains uncertain. We report that hemocytes in the caterpillar, Pieris rapae are able to synthesize 5-HT following activation by lipopolysaccharide. The inhibition of a serotonin-generating enzyme with either pharmacological blockade or RNAi knock-down impaired hemocyte phagocytosis. Biochemical and functional experiments showed that naive hemocytes primarily express 5-HT1B and 5-HT2B receptors. The blockade of 5-HT1B significantly reduced phagocytic ability; however, the blockade of 5-HT2B increased hemocyte phagocytosis. The 5-HT1B-null Drosophila melanogaster mutants showed higher mortality than controls when infected with bacteria, due to their decreased phagocytotic ability. Flies expressing 5-HT1B or 5-HT2B RNAi in hemocytes also showed similar sensitivity to infection. Combined, these data demonstrate that 5-HT mediates hemocyte phagocytosis through 5-HT1B and 5-HT2B receptors and serotonergic signaling performs critical modulatory functions in immune systems of animals separated by 500 million years of evolution.

SUBMITTER: Qi YX 

PROVIDER: S-EPMC4829436 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature

altmetric image

Publications

Serotonin modulates insect hemocyte phagocytosis via two different serotonin receptors.

Qi Yi-Xiang YX   Huang Jia J   Li Meng-Qi MQ   Wu Ya-Su YS   Xia Ren-Ying RY   Ye Gong-Yin GY  

eLife 20160314


Serotonin (5-HT) modulates both neural and immune responses in vertebrates, but its role in insect immunity remains uncertain. We report that hemocytes in the caterpillar, Pieris rapae are able to synthesize 5-HT following activation by lipopolysaccharide. The inhibition of a serotonin-generating enzyme with either pharmacological blockade or RNAi knock-down impaired hemocyte phagocytosis. Biochemical and functional experiments showed that naive hemocytes primarily express 5-HT1B and 5-HT2B rece  ...[more]

Similar Datasets

| S-EPMC4503959 | biostudies-literature
| S-EPMC9103294 | biostudies-literature
2020-07-18 | PXD019317 | Pride
| S-EPMC7476716 | biostudies-literature
| S-EPMC6280549 | biostudies-other
| S-EPMC3663549 | biostudies-literature
| S-EPMC5587260 | biostudies-literature
| S-EPMC11003224 | biostudies-literature
| S-EPMC3174222 | biostudies-literature
| S-EPMC5102739 | biostudies-literature