Ontology highlight
ABSTRACT:
SUBMITTER: Ayub M
PROVIDER: S-EPMC4830132 | biostudies-literature | 2015 Aug
REPOSITORIES: biostudies-literature
Ayub Mariam M Stoddart David D Bayley Hagan H
ACS nano 20150728 8
The α-hemolysin (αHL) protein nanopore has been investigated previously as a base detector for the strand sequencing of DNA and RNA. Recent findings have suggested that shorter pores might provide improved base discrimination. New work has also shown that truncated-barrel mutants (TBM) of αHL form functional pores in lipid bilayers. Therefore, we tested TBM pores for the ability to recognize bases in DNA strands immobilized within them. In the case of TBMΔ6, in which the barrel is shortened by ∼ ...[more]