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Unzipping of Double-stranded DNA in Engineered ?-Hemolysin Pores.

ABSTRACT: Biological protein ?-hemolysin nanopore is under intense investigation as a potential platform for rapid and low-cost DNA sequencing. However, due to its narrow constriction, analysis of DNA in the ?-hemolysin pore has long time been restricted to single strands. In this paper, we report that by introducing new surface functional groups into the ?-hemolysin pore, facilitated unzipping of double-stranded DNA through the channel could be achieved. Since the mean residence time of the DNA events is dependent on the length of the duplex, and also varies with the nucleotide base composition, the modified protein pore approach offers the potential for rapid double-stranded DNA analysis, including sequencing.

SUBMITTER: Liu A

PROVIDER: S-EPMC3119559 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature

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Unzipping of Double-stranded DNA in Engineered α-Hemolysin Pores.

Liu Aihua A Zhao Qitao Q Krishantha D M Milan DM Guan Xiyun X

The journal of physical chemistry letters 20110601 12

Biological protein α-hemolysin nanopore is under intense investigation as a potential platform for rapid and low-cost DNA sequencing. However, due to its narrow constriction, analysis of DNA in the α-hemolysin pore has long time been restricted to single strands. In this paper, we report that by introducing new surface functional groups into the α-hemolysin pore, facilitated unzipping of double-stranded DNA through the channel could be achieved. Since the mean residence time of the DNA events is ...[more]

PMID: 21709813

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