Ontology highlight
ABSTRACT:
SUBMITTER: Lin W
PROVIDER: S-EPMC4833397 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
Lin Weilin W Quintero Andres A Zhang Yixin Y
PloS one 20160415 4
The immunosuppressive drug cyclosporin A (CsA) binds to its receptor protein cyclophilin 18 (Cyp18) in two distinct kinetic phases, while the mechanism remains elusive. Stopped-flow measurements coupled with titration and competition experiments were used to investigate the puzzling two-phase process of CsA and Cyp18 interaction. This study leads to the dissection of different conformational fractions of either direct fast binding or slow binding with rate-limiting conformational inter-conversio ...[more]