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Dodine as a transparent protein denaturant for circular dichroism and infrared studies.

ABSTRACT: The fungicide dodine combines the cooperative denaturation properties of guanidine with the mM denaturation activity of SDS. It was previously tested only on two small model proteins. Here we show that it can be used as a chemical denaturant for phosphoglycerate kinase (PGK), a much larger two-domain enzyme. In addition to its properties as a chemical denaturant, dodine facilitates thermal denaturation of PGK, and we show for the first time that it also facilitates pressure denaturation of a protein. Much higher quality circular dichroism and amide I' infrared spectra of PGK can be obtained in dodine than in guanidine, opening the possibility for use of dodine as a denaturant when UV or IR detection is desirable. One caution is that dodine denaturation, like other detergent-based denaturants, is less reversible than guanidine denaturation.

SUBMITTER: Guin D

PROVIDER: S-EPMC4838651 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature

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Dodine as a transparent protein denaturant for circular dichroism and infrared studies.

Guin Drishti D Sye Kori K Dave Kapil K Gruebele Martin M

Protein science : a publication of the Protein Society 20160321 5

The fungicide dodine combines the cooperative denaturation properties of guanidine with the mM denaturation activity of SDS. It was previously tested only on two small model proteins. Here we show that it can be used as a chemical denaturant for phosphoglycerate kinase (PGK), a much larger two-domain enzyme. In addition to its properties as a chemical denaturant, dodine facilitates thermal denaturation of PGK, and we show for the first time that it also facilitates pressure denaturation of a pro ...[more]

PMID: 26941080

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