Ontology highlight
ABSTRACT:
SUBMITTER: Budziszewska M
PROVIDER: S-EPMC4839060 | biostudies-literature | 2016 May
REPOSITORIES: biostudies-literature
Budziszewska Marta M Wieczorek Przemysław P Obrępalska-Stęplowska Aleksandra A
Archives of virology 20160218 5
'Torrado' disease caused by tomato torrado virus (ToTV) is responsible for considerable losses in tomato production. Therefore, a one-step reverse transcription loop-mediated isothermal amplification protocol for early and fast detection of ToTV isolates has been developed. The RNA extracted from ToTV-infected plants was tested using this protocol with a set of six primers specific for the Vp35 coat protein gene sequence. The amplified products were analyzed using amplification curves, electroph ...[more]