Ontology highlight
ABSTRACT:
SUBMITTER: Song CX
PROVIDER: S-EPMC4843451 | biostudies-literature | 2016 Apr
REPOSITORIES: biostudies-literature
Song Chun-Xiao CX Diao Jiajie J Brunger Axel T AT Quake Stephen R SR
Proceedings of the National Academy of Sciences of the United States of America 20160328 16
The modifications 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) are the two major DNA epigenetic modifications in mammalian genomes and play crucial roles in development and pathogenesis. Little is known about the colocalization or potential correlation of these two modifications. Here we present an ultrasensitive single-molecule imaging technology capable of detecting and quantifying 5hmC and 5mC from trace amounts of DNA. We used this approach to perform single-molecule fluorescenc ...[more]