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A panel data set on harvest and perfusion decellularization of porcine rectus abdominis.


ABSTRACT: In this dataset, we particularly depicted the harvest and perfusion decellularization of porcine rectus abdominis (RA), accompanied with displaying of the retained vascular trees within the perfusion-decellularized skeletal muscle matrix (pM-ECM) using vascular corrosion casting. In addition, several important tips for successful pM-ECM preparation were emphasized, which including using anatomically isolated skeletal muscle as tissue source with all main feeding and draining vessels perfused, preserving the internal microcirculation availability, aseptic technique and pyrogen free in all steps, sequential perfusion via artery or vein, and longtime washing after decellularization. The data are supplemental to our original research article describing detailed associations of pM-ECM as a clinically relevant scale, three-dimensional scaffold with a vascular network template for tissue-specific regeneration, "Perfusion-decellularized skeletal muscle as a three-dimensional scaffold with a vascular network template" Zhang et al. (2016) [1].

SUBMITTER: Zhang J 

PROVIDER: S-EPMC4845073 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature

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A panel data set on harvest and perfusion decellularization of porcine rectus abdominis.

Zhang Jian J   Cheng Wen Yue WY   Hu Zhi Qian ZQ   Turner Neill J NJ   Zhang Li L   Wang Qiang Q   Badylak Stephen F SF  

Data in brief 20160413


In this dataset, we particularly depicted the harvest and perfusion decellularization of porcine rectus abdominis (RA), accompanied with displaying of the retained vascular trees within the perfusion-decellularized skeletal muscle matrix (pM-ECM) using vascular corrosion casting. In addition, several important tips for successful pM-ECM preparation were emphasized, which including using anatomically isolated skeletal muscle as tissue source with all main feeding and draining vessels perfused, pr  ...[more]

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