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Genetically targeted magnetic control of the nervous system.

ABSTRACT: Optogenetic and chemogenetic actuators are critical for deconstructing the neural correlates of behavior. However, these tools have several limitations, including invasive modes of stimulation or slow on/off kinetics. We have overcome these disadvantages by synthesizing a single-component, magnetically sensitive actuator, "Magneto," comprising the cation channel TRPV4 fused to the paramagnetic protein ferritin. We validated noninvasive magnetic control over neuronal activity by demonstrating remote stimulation of cells using in vitro calcium imaging assays, electrophysiological recordings in brain slices, in vivo electrophysiological recordings in the brains of freely moving mice, and behavioral outputs in zebrafish and mice. As proof of concept, we used Magneto to delineate a causal role of striatal dopamine receptor 1 neurons in mediating reward behavior in mice. Together our results present Magneto as an actuator capable of remotely controlling circuits associated with complex animal behaviors.

SUBMITTER: Wheeler MA

PROVIDER: S-EPMC4846560 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature

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Genetically targeted magnetic control of the nervous system.

Wheeler Michael A MA Smith Cody J CJ Ottolini Matteo M Barker Bryan S BS Purohit Aarti M AM Grippo Ryan M RM Gaykema Ronald P RP Spano Anthony J AJ Beenhakker Mark P MP Kucenas Sarah S Patel Manoj K MK Deppmann Christopher D CD Güler Ali D AD

Nature neuroscience 20160307 5

Optogenetic and chemogenetic actuators are critical for deconstructing the neural correlates of behavior. However, these tools have several limitations, including invasive modes of stimulation or slow on/off kinetics. We have overcome these disadvantages by synthesizing a single-component, magnetically sensitive actuator, "Magneto," comprising the cation channel TRPV4 fused to the paramagnetic protein ferritin. We validated noninvasive magnetic control over neuronal activity by demonstrating rem ...[more]

PMID: 26950006

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Genetically targeted magnetic control of the nervous system.

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Genetically targeted magnetic control of the nervous system.

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