Dataset Information

Robust quantitative scratch assay.

ABSTRACT:

Unlabelled

The wound healing assay (or scratch assay) is a technique frequently used to quantify the dependence of cell motility-a central process in tissue repair and evolution of disease-subject to various treatments conditions. However processing the resulting data is a laborious task due its high throughput and variability across images. This Robust Quantitative Scratch Assay algorithm introduced statistical outputs where migration rates are estimated, cellular behaviour is distinguished and outliers are identified among groups of unique experimental conditions. Furthermore, the RQSA decreased measurement errors and increased accuracy in the wound boundary at comparable processing times compared to previously developed method (TScratch).

Availability and implementation

The RQSA is freely available at: http://ophid.utoronto.ca/RQSA/RQSA_Scripts.zip The image sets used for training and validation and results are available at: (http://ophid.utoronto.ca/RQSA/trainingSet.zip, http://ophid.utoronto.ca/RQSA/validationSet.zip, http://ophid.utoronto.ca/RQSA/ValidationSetResults.zip, http://ophid.utoronto.ca/RQSA/ValidationSet_H1975.zip, http://ophid.utoronto.ca/RQSA/ValidationSet_H1975Results.zip, http://ophid.utoronto.ca/RQSA/RobustnessSet.zip, http://ophid.utoronto.ca/RQSA/RobustnessSet.zip). Supplementary Material is provided for detailed description of the development of the RQSA.

Contact

juris@ai.utoronto.ca

Supplementary information

Supplementary data are available at Bioinformatics online.

SUBMITTER: Vargas A

PROVIDER: S-EPMC4848397 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Robust quantitative scratch assay.

Vargas Andrea A Angeli Marc M Pastrello Chiara C McQuaid Rosanne R Li Han H Jurisicova Andrea A Jurisica Igor I

Bioinformatics (Oxford, England) 20151231 9

<h4>Unlabelled</h4>The wound healing assay (or scratch assay) is a technique frequently used to quantify the dependence of cell motility-a central process in tissue repair and evolution of disease-subject to various treatments conditions. However processing the resulting data is a laborious task due its high throughput and variability across images. This Robust Quantitative Scratch Assay algorithm introduced statistical outputs where migration rates are estimated, cellular behaviour is distingui ...[more]

PMID: 26722119

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Dataset Information

Robust quantitative scratch assay.

Unlabelled

Availability and implementation

Contact

Supplementary information

Publications

Robust quantitative scratch assay.

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