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Accurate Extraction of Nanometer Distances in Multimers by Pulse EPR.

ABSTRACT: Pulse electron paramagnetic resonance (EPR) is gaining increasing importance in structural biology. The PELDOR (pulsed electron-electron double resonance) method allows extracting distance information on the nanometer scale. Here, we demonstrate the efficient extraction of distances from multimeric systems such as membrane-embedded ion channels where data analysis is commonly hindered by multi-spin effects.

SUBMITTER: Valera S

PROVIDER: S-EPMC4848767 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature

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Accurate Extraction of Nanometer Distances in Multimers by Pulse EPR.

Valera Silvia S Ackermann Katrin K Pliotas Christos C Huang Hexian H Naismith James H JH Bode Bela E BE

Chemistry (Weinheim an der Bergstrasse, Germany) 20160225 14

Pulse electron paramagnetic resonance (EPR) is gaining increasing importance in structural biology. The PELDOR (pulsed electron-electron double resonance) method allows extracting distance information on the nanometer scale. Here, we demonstrate the efficient extraction of distances from multimeric systems such as membrane-embedded ion channels where data analysis is commonly hindered by multi-spin effects. ...[more]

PMID: 26865468

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