Dataset Information

Molecular Characterization of MaCCS, a Novel Copper Chaperone Gene Involved in Abiotic and Hormonal Stress Responses in Musa acuminata cv. Tianbaojiao.

ABSTRACT: Copper/zinc superoxide dismutases (Cu/ZnSODs) play important roles in improving banana resistance to adverse conditions, but their activities depend on the copper chaperone for superoxide dismutase (CCS) delivering copper to them. However, little is known about CCS in monocots and under stress conditions. Here, a novel CCS gene (MaCCS) was obtained from a banana using reverse transcription PCR and rapid-amplification of cDNA ends (RACE) PCR. Sequence analyses showed that MaCCS has typical CCS domains and a conserved gene structure like other plant CCSs. Alternative transcription start sites (ATSSs) and alternative polyadenylation contribute to the mRNA diversity of MaCCS. ATSSs in MaCCS resulted in one open reading frame containing two in-frame start codons to form two protein versions, which is supported by the MaCCS subcellular localization of in both cytosol and chloroplasts. Furthermore, MaCCS promoter was found to contain many cis-elements associated with abiotic and hormonal responses. Quantitative real-time PCR analysis showed that MaCCS was expressed in all tested tissues (leaves, pseudostems and roots). In addition, MaCCS expression was significantly induced by light, heat, drought, abscisic acid and indole-3-acetic acid, but inhibited by relatively high concentrations of CuSO? and under cold treatment, which suggests that MaCCS is involved in abiotic and hormonal responses.

SUBMITTER: Feng X

PROVIDER: S-EPMC4848897 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature

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Molecular Characterization of MaCCS, a Novel Copper Chaperone Gene Involved in Abiotic and Hormonal Stress Responses in Musa acuminata cv. Tianbaojiao.

Feng Xin X Chen Fanglan F Liu Weihua W Thu Min Kyaw MK Zhang Zihao Z Chen Yukun Y Cheng Chunzhen C Lin Yuling Y Wang Tianchi T Lai Zhongxiong Z

International journal of molecular sciences 20160324 4

Copper/zinc superoxide dismutases (Cu/ZnSODs) play important roles in improving banana resistance to adverse conditions, but their activities depend on the copper chaperone for superoxide dismutase (CCS) delivering copper to them. However, little is known about CCS in monocots and under stress conditions. Here, a novel CCS gene (MaCCS) was obtained from a banana using reverse transcription PCR and rapid-amplification of cDNA ends (RACE) PCR. Sequence analyses showed that MaCCS has typical CCS do ...[more]

PMID: 27023517

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Project description:The salinization of soil is a widespread environmental problem. Banana (Musa acuminata L.) is a salt-sensitive plant whose growth, development, and production are constrained by salt stresses. However, the tolerance mechanism of this salt-sensitive banana to salt stress is still unclear. This study aimed to investigate the influence of NaCl treatment on phenotypic, physiological, and transcriptome changes in bananas. We found that the content of root activity, MDA, Pro, soluble sugar, soluble protein, and antioxidant enzymes activity in salt-stress treatment were significantly higher than the control in bananas. Transcriptome sequencing result identified an overall of 3,378 differentially expressed genes (DEGs) in banana leaves, and the Kyoto Encyclopedia of Genes and Genomes analysis indicated that these DEGs were involved in phenylpropanoid biosynthesis process, ribosome process, starch and sucrose metabolism, amino sugar process, and plant hormone signal transduction process that had simultaneously changed their expression under salt stress, which indicated these DEGs may play a role in promoting BD banana growth under salt treatments. The genes which were enriched in the phenylpropanoid biosynthesis process, starch and sucrose metabolism process, amino sugar process, and plant hormone signal transduction process were specifically regulated to respond to the salt stress treatments. Here, totally 48 differentially expressed transcription factors (TFs), including WRKY, MYB, NAC, and bHLH, were annotated in BD banana under salt stress. In the phenylpropane biosynthesis pathway, all transcripts encoding key enzymes were found to be significantly up-regulated, indicating that the genes in these pathways may play a significant function in the response of BD banana to salt stress. In conclusion, this study provides new insights into the mechanism of banana tolerance to salt stress, which provides a potential application for the genetic improvement of banana with salt tolerance.

Project description:Superoxide dismutase (SOD) is an essential enzyme of the plant antioxidant system that responds to oxidative stresses caused by adverse conditions. Banana is an important staple and economic crop in tropical and subtropical regions. However, its growth and yield are constantly affected by various abiotic stresses. To analyze the roles of distinct SOD genes under various stresses, a detailed characterization and analysis of the SOD gene family in Cavendish banana is indispensable.The presence and structure of the SOD family genes were experimentally verified using 5'/3' RACE-PCR, reverse transcription PCR and PCR. Then, their syntenic relationships, conserved motifs and phylogenetic relationships were analyzed using software. Cis-elements present in the promoters were predicted via PlantCARE. And the expression levels under abiotic and hormonal stresses were determined using real-time quantitative polymerase chain reaction.In total, 25 'Tianbaojiao' SOD cDNAs (MaSODs), which encoded six Cu/ZnSODs, four MnSODs and two FeSODs, were cloned. The 12 MaSOD genes were divided into four groups based on their conserved motifs, which corroborated their classifications based on gene-structure patterns and subcellular localizations. Eleven MaSOD promoters were isolated and found to contain many cis-acting elements involved in stress responses. Gene expression analysis showed that 11 out of the 12 MaSODs were expressed in all tested tissues (leaf, pseudostem and root), whereas MaCSD2B was expressed only in leaves and roots. Specific MaSOD members exhibited different expression patterns under abiotic and hormonal treatments. Among the 12 MaSOD genes, MaCSD1D was the only one that responded to all eight treatments, suggesting that this gene plays a predominant role in reactive oxygen species scavenging caused by various stresses in banana.A genome-wide analysis showed that the 'Tianbaojiao' banana harbored an expanded SOD gene family. Whole genome duplication, segmental duplication and complex transcriptional regulation contributed to the gene expansion and mRNA diversity of the MaSODs. The expression patterns of distinct MaSOD genes showed that they are important responses to different abiotic and hormonal stresses in banana.

Dataset Information

Molecular Characterization of MaCCS, a Novel Copper Chaperone Gene Involved in Abiotic and Hormonal Stress Responses in Musa acuminata cv. Tianbaojiao.

Publications

Molecular Characterization of MaCCS, a Novel Copper Chaperone Gene Involved in Abiotic and Hormonal Stress Responses in Musa acuminata cv. Tianbaojiao.

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