Floating Droplet Array: An Ultrahigh-Throughput Device for Droplet Trapping, Real-time Analysis and Recovery.
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ABSTRACT:
SUBMITTER: Labanieh L
PROVIDER: S-EPMC4849166 | biostudies-literature | 2015 Oct
REPOSITORIES: biostudies-literature
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Floating Droplet Array: An Ultrahigh-Throughput Device for Droplet Trapping, Real-time Analysis and Recovery.
Labanieh Louai L Nguyen Thi N TN Zhao Weian W Kang Dong-Ku DK
Micromachines 20150930 10
We describe the design, fabrication and use of a dual-layered microfluidic device for ultrahigh-throughput droplet trapping, analysis, and recovery using droplet buoyancy. To demonstrate the utility of this device for digital quantification of analytes, we quantify the number of droplets, which contain a β-galactosidase-conjugated bead among more than 100,000 immobilized droplets. In addition, we demonstrate that this device can be used for droplet clustering and real-time analysis by clustering ...[more]
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