Floating Droplet Array: An Ultrahigh-Throughput Device for Droplet Trapping, Real-time Analysis and Recovery.

ABSTRACT: We describe the design, fabrication and use of a dual-layered microfluidic device for ultrahigh-throughput droplet trapping, analysis, and recovery using droplet buoyancy. To demonstrate the utility of this device for digital quantification of analytes, we quantify the number of droplets, which contain a ?-galactosidase-conjugated bead among more than 100,000 immobilized droplets. In addition, we demonstrate that this device can be used for droplet clustering and real-time analysis by clustering several droplets together into microwells and monitoring diffusion of fluorescein, a product of the enzymatic reaction of ?-galactosidase and its fluorogenic substrate FDG, between droplets.

SUBMITTER: Labanieh L 

PROVIDER: S-EPMC4849166 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature