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Potency of a thermostabilised chimpanzee adenovirus Rift Valley Fever vaccine in cattle.

ABSTRACT: Development of safe and efficacious vaccines whose potency is unaffected by long-term storage at ambient temperature would obviate major vaccine deployment hurdles and limit wastage associated with breaks in the vaccine cold chain. Here, we evaluated the immunogenicity of a novel chimpanzee adenovirus vectored Rift Valley Fever vaccine (ChAdOx1-GnGc) in cattle, following its thermostabilisation by slow desiccation on glass fiber membranes in the non-reducing sugars trehalose and sucrose. Thermostabilised ChAdOx1-GnGc vaccine stored for 6 months at 25, 37 or 45 ° C elicited comparable Rift Valley Fever virus neutralising antibody titres to those elicited by the 'cold chain' vaccine (stored at -80 ° C throughout) at the same dose, and these were within the range associated with protection against Rift Valley Fever in cattle. The results support the use of sugar-membrane thermostabilised vaccines in target livestock species.

SUBMITTER: Dulal P

PROVIDER: S-EPMC4851241 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature

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Potency of a thermostabilised chimpanzee adenovirus Rift Valley Fever vaccine in cattle.

Dulal Pawan P Wright Daniel D Ashfield Rebecca R Hill Adrian V S AV Charleston Bryan B Warimwe George M GM

Vaccine 20160325 20

Development of safe and efficacious vaccines whose potency is unaffected by long-term storage at ambient temperature would obviate major vaccine deployment hurdles and limit wastage associated with breaks in the vaccine cold chain. Here, we evaluated the immunogenicity of a novel chimpanzee adenovirus vectored Rift Valley Fever vaccine (ChAdOx1-GnGc) in cattle, following its thermostabilisation by slow desiccation on glass fiber membranes in the non-reducing sugars trehalose and sucrose. Thermos ...[more]

PMID: 27020712

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