Microwell arrays reveal cellular heterogeneity during the clonal expansion of transformed human cells.
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ABSTRACT:
SUBMITTER: Chang TC
PROVIDER: S-EPMC4854201 | biostudies-literature | 2015 Dec
REPOSITORIES: biostudies-literature
Publications
Microwell arrays reveal cellular heterogeneity during the clonal expansion of transformed human cells.
Chang Tim C TC Tang Weiliang W Koh William Jen Hoe WJ Rettie Alexander J E AJ Emond Mary J MJ Monnat Raymond J RJ Folch Albert A
Technology 20151201 4
We developed micromolded microwell arrays to study the proliferation and senescence of single cells. Microwell arrays were designed to be compatible with conventional cell culture protocols to simplify cell loading, cell culture, and imaging. We demonstrated the utility of these arrays by measuring the proliferation and senescence of isogenic cells which expressed or had been depleted of the human Werner syndrome protein. Our results allowed us to reveal cell-to-cell heterogeneity in proliferati ...[more]
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