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The Myb domain of LUX ARRHYTHMO in complex with DNA: expression, purification and crystallization.

ABSTRACT: LUX ARRHYTHMO (LUX) is a Myb-domain transcription factor that plays an important role in regulating the circadian clock. Lux mutations cause severe clock defects and arrhythmia in constant light and dark. In order to examine the molecular mechanisms underlying the function of LUX, the DNA-binding Myb domain was cloned, expressed and purified. The DNA-binding activity of the Myb domain was confirmed using electrophoretic mobility shift assays (EMSAs), demonstrating that the LUX Myb domain is able to bind to DNA with nanomolar affinity. In order to investigate the specificity determinants of protein-DNA interactions, the protein was co-crystallized with a 10-mer cognate DNA. Initial crystallization results for the selenomethionine-derivatized protein and data-set collection statistics are reported. Data collection was performed using the MeshAndCollect workflow available at the ESRF.

SUBMITTER: Silva CS

PROVIDER: S-EPMC4854562 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature

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The Myb domain of LUX ARRHYTHMO in complex with DNA: expression, purification and crystallization.

Silva Catarina S CS Lai Xuelei X Nanao Max M Zubieta Chloe C

Acta crystallographica. Section F, Structural biology communications 20160422 Pt 5

LUX ARRHYTHMO (LUX) is a Myb-domain transcription factor that plays an important role in regulating the circadian clock. Lux mutations cause severe clock defects and arrhythmia in constant light and dark. In order to examine the molecular mechanisms underlying the function of LUX, the DNA-binding Myb domain was cloned, expressed and purified. The DNA-binding activity of the Myb domain was confirmed using electrophoretic mobility shift assays (EMSAs), demonstrating that the LUX Myb domain is able ...[more]

PMID: 27139826

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