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Dose-dependent fate of GFP-expressing Escherichia coli in the alimentary canal of adult house flies.

ABSTRACT: The adult house fly Musca domestica (L.) (Diptera: Muscidae) can disseminate bacteria from microbe-rich substrates to areas in which humans and domesticated animals reside. Because bacterial abundance fluctuates widely across substrates, flies encounter and ingest varying amounts of bacteria. This study investigated the dose-dependent survival of bacteria in house flies. Flies were fed four different 'doses' of green fluorescent protein (GFP)-expressing Escherichia coli (GFP E.?coli) (very low, low, medium, high) and survival was determined at 1, 4, 10 and 22?h post-ingestion by culture and epifluorescent microscopy. Over 22?h, the decline in GFP E.?coli was significant in all treatments (P?

SUBMITTER: Kumar NH

PROVIDER: S-EPMC4856564 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature

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Dose-dependent fate of GFP-expressing Escherichia coli in the alimentary canal of adult house flies.

Kumar N H V NH Nayduch D D

Medical and veterinary entomology 20160204 2

The adult house fly Musca domestica (L.) (Diptera: Muscidae) can disseminate bacteria from microbe-rich substrates to areas in which humans and domesticated animals reside. Because bacterial abundance fluctuates widely across substrates, flies encounter and ingest varying amounts of bacteria. This study investigated the dose-dependent survival of bacteria in house flies. Flies were fed four different 'doses' of green fluorescent protein (GFP)-expressing Escherichia coli (GFP E. coli) (very low, ...[more]

PMID: 26843509

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Project description:Multifunctional novel core-shell composites, CdSNPs@ZIF-8, have been synthesized by in situ encapsulation of different amounts of CdSNPs (150, 300, and 500 μL suspension of CdSNPs in methanol) in ZIF-8 at room temperature. These composites have been characterized by powder X-ray diffraction, X-ray photoelectron spectroscopy (XPS), field emission scanning electron microscopy, high-resolution transmission electron microscopy (HRTEM), and diffuse reflectance spectroscopy techniques and Brunauer-Emmett-Teller surface analysis. XPS and HRTEM indicate the encapsulation of CdSNPs within ZIF-8 crystal without disturbing the crystal order of ZIF-8. The average size of embedded CdSNPs (determined by the particle size distribution from HRTEM) is found to be 16.34 nm. CdSNPs@ZIF-8 showed potential to be used as an antibacterial agent against the broad spectrum of bacterial strains such as Gram-positive Staphylococcus aureus and Gram-negative green fluorescent protein-expressing Escherichia coli in aqueous medium, as evident by various biophysical experiments, viz., 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, optical density and fluorescence spectroscopic studies, fluorescence and optical microscopic image analysis, disk diffusion assay, field emission scanning electron microscopy, and flow cytometry for reactive oxygen species induction assay. Further, the composite has been used as an efficient photocatalyst for the degradation of organic pollutants, such as methylene blue dye, in aqueous medium and found that the core-shell composite, CdSNPs@ZIF-8 (150 μL) (abbreviated as NC-1) (5 mg), exhibited higher photocatalytic activity (≈1.8 times) than CdSNPs for degradation of 90% of methylene blue (10 mL of 10 ppm) at pH ≥ 7 due to the synergetic effect. Therefore, in situ encapsulation of CdSNPs in ZIF-8 provides an easy executable measure for purification of wastewater effluents for the effective photocatalytic degradation of organic pollutants as well as to remove the bacterial contamination under sunlight.

Project description:BackgroundSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a recently emerged coronavirus that is the causative agent of the coronavirus disease 2019 (COVID-19) pandemic. COVID-19 in humans is characterized by a wide range of symptoms that range from asymptomatic to mild or severe illness including death. SARS-CoV-2 is highly contagious and is transmitted via the oral-nasal route through droplets and aerosols, or through contact with contaminated fomites. House flies are known to transmit bacterial, parasitic and viral diseases to humans and animals as mechanical vectors. Previous studies have shown that house flies can mechanically transmit coronaviruses, such as turkey coronavirus; however, the house fly's role in SARS-CoV-2 transmission has not yet been explored. The goal of this work was to investigate the potential of house flies to mechanically transmit SARS-CoV-2. For this purpose, it was determined whether house flies can acquire SARS-CoV-2, harbor live virus and mechanically transmit the virus to naive substrates and surfaces.MethodsTwo independent studies were performed to address the study objectives. In the first study, house flies were tested for infectivity after exposure to SARS-CoV-2-spiked medium or milk. In the second study, environmental samples were tested for infectivity after contact with SARS-CoV-2-exposed flies. During both studies, samples were collected at various time points post-exposure and evaluated by SARS-CoV-2-specific RT-qPCR and virus isolation.ResultsAll flies exposed to SARS-CoV-2-spiked media or milk substrates were positive for viral RNA at 4 h and 24 h post-exposure. Infectious virus was isolated only from the flies exposed to virus-spiked milk but not from those exposed to virus-spiked medium. Moreover, viral RNA was detected in environmental samples after contact with SARS-CoV-2 exposed flies, although no infectious virus was recovered from these samples.ConclusionsUnder laboratory conditions, house flies acquired and harbored infectious SARS-CoV-2 for up to 24 h post-exposure. In addition, house flies were able to mechanically transmit SARS-CoV-2 genomic RNA to the surrounding environment up to 24 h post-exposure. Further studies are warranted to determine if house fly transmission occurs naturally and the potential public health implications of such events.

Dataset Information

Dose-dependent fate of GFP-expressing Escherichia coli in the alimentary canal of adult house flies.

Publications

Dose-dependent fate of GFP-expressing Escherichia coli in the alimentary canal of adult house flies.

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