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The RNA Helicase eIF4A Is Required for Sapovirus Translation.

ABSTRACT: The eukaryotic initiation factor 4A (eIF4A) is a DEAD box helicase that unwinds RNA structure in the 5' untranslated region (UTR) of mRNAs. Here, we investigated the role of eIF4A in porcine sapovirus VPg-dependent translation. Using inhibitors and dominant-negative mutants, we found that eIF4A is required for viral translation and infectivity, suggesting that despite the presence of a very short 5' UTR, eIF4A is required to unwind RNA structure in the sapovirus genome to facilitate virus translation.

SUBMITTER: Hosmillo M

PROVIDER: S-EPMC4859703 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature

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The RNA Helicase eIF4A Is Required for Sapovirus Translation.

Hosmillo Myra M Sweeney Trevor R TR Chaudhry Yasmin Y Leen Eoin E Curry Stephen S Goodfellow Ian I Cho Kyoung-Oh KO

Journal of virology 20160429 10

The eukaryotic initiation factor 4A (eIF4A) is a DEAD box helicase that unwinds RNA structure in the 5' untranslated region (UTR) of mRNAs. Here, we investigated the role of eIF4A in porcine sapovirus VPg-dependent translation. Using inhibitors and dominant-negative mutants, we found that eIF4A is required for viral translation and infectivity, suggesting that despite the presence of a very short 5' UTR, eIF4A is required to unwind RNA structure in the sapovirus genome to facilitate virus transl ...[more]

PMID: 26937032

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