Unknown

Dataset Information

0

A Glial K/Cl Transporter Controls Neuronal Receptive Ending Shape by Chloride Inhibition of an rGC.


ABSTRACT: Neurons receive input from the outside world or from other neurons through neuronal receptive endings (NREs). Glia envelop NREs to create specialized microenvironments; however, glial functions at these sites are poorly understood. Here, we report a molecular mechanism by which glia control NRE shape and associated animal behavior. The C. elegans AMsh glial cell ensheathes the NREs of 12 neurons, including the thermosensory neuron AFD. KCC-3, a K/Cl transporter, localizes specifically to a glial microdomain surrounding AFD receptive ending microvilli, where it regulates K(+) and Cl(-) levels. We find that Cl(-) ions function as direct inhibitors of an NRE-localized receptor-guanylyl-cyclase, GCY-8, which synthesizes cyclic guanosine monophosphate (cGMP). High cGMP mediates the effects of glial KCC-3 on AFD shape by antagonizing the actin regulator WSP-1/NWASP. Components of this pathway are broadly expressed throughout the nervous system, suggesting that ionic regulation of the NRE microenvironment may be a conserved mechanism by which glia control neuron shape and function.

SUBMITTER: Singhvi A 

PROVIDER: S-EPMC4860081 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature

altmetric image

Publications

A Glial K/Cl Transporter Controls Neuronal Receptive Ending Shape by Chloride Inhibition of an rGC.

Singhvi Aakanksha A   Liu Bingqian B   Friedman Christine J CJ   Fong Jennifer J   Lu Yun Y   Huang Xin-Yun XY   Shaham Shai S  

Cell 20160407 4


Neurons receive input from the outside world or from other neurons through neuronal receptive endings (NREs). Glia envelop NREs to create specialized microenvironments; however, glial functions at these sites are poorly understood. Here, we report a molecular mechanism by which glia control NRE shape and associated animal behavior. The C. elegans AMsh glial cell ensheathes the NREs of 12 neurons, including the thermosensory neuron AFD. KCC-3, a K/Cl transporter, localizes specifically to a glial  ...[more]

Similar Datasets

| S-EPMC2196133 | biostudies-literature
| S-EPMC2453132 | biostudies-literature
| S-EPMC5431760 | biostudies-literature
| S-EPMC8481871 | biostudies-literature
| S-EPMC2833346 | biostudies-literature
| S-EPMC6592889 | biostudies-literature
| S-EPMC2898430 | biostudies-literature
| S-EPMC4162178 | biostudies-literature
| S-EPMC3672097 | biostudies-literature
| S-EPMC2758809 | biostudies-other