Ontology highlight
ABSTRACT:
SUBMITTER: Kursan S
PROVIDER: S-EPMC5431760 | biostudies-literature | 2017 May
REPOSITORIES: biostudies-literature
Kursan Shams S McMillen Timothy S TS Beesetty Pavani P Dias-Junior Eduardo E Almutairi Mohammed M MM Sajib Abu A AA Kozak J Ashot JA Aguilar-Bryan Lydia L Di Fulvio Mauricio M
Scientific reports 20170511 1
Intracellular chloride concentration ([Cl<sup>-</sup>]<sub>i</sub>) in pancreatic β-cells is kept above electrochemical equilibrium due to the predominant functional presence of Cl<sup>-</sup> loaders such as the Na<sup>+</sup>K<sup>+</sup>2Cl<sup>-</sup> co-transporter 1 (Slc12a2) over Cl<sup>-</sup>extruders of unidentified nature. Using molecular cloning, RT-PCR, Western blotting, immunolocalization and in vitro functional assays, we establish that the "neuron-specific" K<sup>+</sup>Cl<sup>-< ...[more]