Ontology highlight
ABSTRACT:
SUBMITTER: Beilsten-Edmands V
PROVIDER: S-EPMC4860841 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Beilsten-Edmands Victoria V Gordiyenko Yuliya Y Kung Jocky Ck JC Mohammed Shabaz S Schmidt Carla C Robinson Carol V CV
Cell discovery 20150811
Translation of messenger RNA (mRNA) into proteins is key to eukaryotic gene expression and begins when initiation factor-2 (eIF2) delivers methionyl initiator tRNA (Met-tRNAi (Met)) to ribosomes. This first step is controlled by eIF2B mediating guanine nucleotide exchange on eIF2. We isolated eIF2 from yeast and used mass spectrometry to study the intact complex, and found that eIF2β is the most labile of the three subunits (eIF2α/β/γ). We then compared conformational dynamics of the ternary com ...[more]