Ontology highlight
ABSTRACT:
SUBMITTER: Motion CL
PROVIDER: S-EPMC4863198 | biostudies-literature | 2016 Apr
REPOSITORIES: biostudies-literature
Motion Claire L CL Lovett Janet E JE Bell Stacey S Cassidy Scott L SL Cruickshank Paul A S PA Bolton David R DR Hunter Robert I RI El Mkami Hassane H Van Doorslaer Sabine S Smith Graham M GM
The journal of physical chemistry letters 20160401 8
This work demonstrates the feasibility of making sensitive nanometer distance measurements between Fe(III) heme centers and nitroxide spin labels in proteins using the double electron-electron resonance (DEER) pulsed EPR technique at 94 GHz. Techniques to measure accurately long distances in many classes of heme proteins using DEER are currently strongly limited by sensitivity. In this paper we demonstrate sensitivity gains of more than 30 times compared with previous lower frequency (X-band) DE ...[more]