Whole-cell conversion of l-glutamic acid into gamma-aminobutyric acid by metabolically engineered Escherichia coli.
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ABSTRACT:
SUBMITTER: Ke C
PROVIDER: S-EPMC4864792 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
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Whole-cell conversion of l-glutamic acid into gamma-aminobutyric acid by metabolically engineered Escherichia coli.
Ke Chongrong C Yang Xinwei X Rao Huanxin H Zeng Wenchao W Hu Meirong M Tao Yong Y Huang Jianzhong J
SpringerPlus 20160511
A simple and high efficient way for the synthesis of gamma-aminobutyric acid (GABA) was developed by using engineered Escherichia coli as whole-cell biocatalyst from l-glutamic acid (l-Glu). Codon optimization of Lactococcus lactis GadB showed the best performance on GABA production when middle copy-number plasmid was used as expression vector in E. coli BW25113. The highest production of GABA reached 308.96 g L(-1) with 99.9 mol% conversion within 12 h, when E. coli ΔgabAB (pRB-lgadB) concentra ...[more]
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