Ontology highlight
ABSTRACT:
SUBMITTER: Sin C
PROVIDER: S-EPMC4868333 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
Sin Celine C Chiarugi Davide D Valleriani Angelo A
PloS one 20160516 5
Pulse-chase experiments are often used to study the degradation of macromolecules such as proteins or mRNA. Considerations for the choice of pulse length include the toxicity of the pulse to the cell and maximization of labeling. In the general case of non-exponential decay, varying the length of the pulse results in decay patterns that look different. Analysis of these patterns without consideration to pulse length would yield incorrect degradation parameters. Here we propose a method that cons ...[more]