Changes in beta cell function occur in prediabetes and early disease in the Lepr (db) mouse model of diabetes.

ABSTRACT:

Aims/hypothesis

Type 2 diabetes is a progressive disease that increases morbidity and the risk of premature death. Glucose dysregulation, such as elevated fasting blood glucose, is observed prior to diabetes onset. A decline in beta cell insulin secretion contributes to the later stages of diabetes, but it is not known what, if any, functional beta cell changes occur in prediabetes and early disease.

Methods

The Lepr (db) mouse (age 13-18 weeks) was used as a model of type 2 diabetes and a two-photon granule fusion assay was used to characterise the secretory response of pancreatic beta cells.

Results

We identified a prediabetic state in db/db mice where the animals responded normally to a glucose challenge but have elevated fasting blood glucose. Isolated islets from

SUBMITTER: Do OH 

PROVIDER: S-EPMC4869737 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature