Ontology highlight
ABSTRACT:
SUBMITTER: Kulkarni M
PROVIDER: S-EPMC4872093 | biostudies-literature | 2016 May
REPOSITORIES: biostudies-literature
Kulkarni Manasi M Nirwan Neha N van Aelst Kara K Szczelkun Mark D MD Saikrishnan Kayarat K
Nucleic acids research 20160314 9
Engineering restriction enzymes with new sequence specificity has been an unaccomplished challenge, presumably because of the complexity of target recognition. Here we report detailed analyses of target recognition by Type ISP restriction-modification enzymes. We determined the structure of the Type ISP enzyme LlaGI bound to its target and compared it with the previously reported structure of a close homologue that binds to a distinct target, LlaBIII. The comparison revealed that, although the t ...[more]