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The scanning electron microscope in microbiology and diagnosis of infectious disease.

ABSTRACT: Despite being an excellent tool for investigating ultrastructure, scanning electron microscopy (SEM) is less frequently used than transmission electron microscopy for microbes such as viruses or bacteria. Here we describe rapid methods that allow SEM imaging of fully hydrated, unfixed microbes without using conventional sample preparation methods. We demonstrate improved ultrastructural preservation, with greatly reduced dehydration and shrinkage, for specimens including bacteria and viruses such as Ebola virus using infiltration with ionic liquid on conducting filter substrates for SEM.

SUBMITTER: Golding CG

PROVIDER: S-EPMC4876401 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature

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The scanning electron microscope in microbiology and diagnosis of infectious disease.

Golding Christine G CG Lamboo Lindsey L LL Beniac Daniel R DR Booth Timothy F TF

Scientific reports 20160523

Despite being an excellent tool for investigating ultrastructure, scanning electron microscopy (SEM) is less frequently used than transmission electron microscopy for microbes such as viruses or bacteria. Here we describe rapid methods that allow SEM imaging of fully hydrated, unfixed microbes without using conventional sample preparation methods. We demonstrate improved ultrastructural preservation, with greatly reduced dehydration and shrinkage, for specimens including bacteria and viruses suc ...[more]

PMID: 27212232

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