Ontology highlight
ABSTRACT:
SUBMITTER: Nii T
PROVIDER: S-EPMC4876534 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
Nii Takenobu T Kohara Hiroshi H Marumoto Tomotoshi T Sakuma Tetsushi T Yamamoto Takashi T Tani Kenzaburo K
BioResearch open access 20160501 1
Efficient gene transfer into human pluripotent stem cells (hPSCs) holds great promise for regenerative medicine and pharmaceutical development. In the past decade, various methods were developed for gene transfer into hPSCs; however, hPSCs form tightly packed colonies, making gene transfer difficult. In this study, we established a stable culture method of hPSCs at a single-cell state to reduce cell density and investigated gene transfection efficiency followed by gene editing efficiency. hPSCs ...[more]