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A Rapid Method for Refolding Cell Surface Receptors and Ligands.

ABSTRACT: Production of membrane-associated cell surface receptors and their ligands is often a cumbersome, expensive, and time-consuming process that limits detailed structural and functional characterization of this important class of proteins. Here we report a rapid method for refolding inclusion-body-based, recombinant cell surface receptors and ligands in one day, a speed equivalent to that of soluble protein production. This method efficiently couples modular on-column immobilized metal ion affinity purification and solid-phase protein refolding. We demonstrated the general utility of this method for producing multiple functionally active immunoreceptors, ligands, and viral decoys, including challenging cell surface proteins that cannot be produced using typical dialysis- or dilution-based refolding approaches.

SUBMITTER: Zhai L

PROVIDER: S-EPMC4877712 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature

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A Rapid Method for Refolding Cell Surface Receptors and Ligands.

Zhai Lu L Wu Ling L Li Feng F Burnham Robert S RS Pizarro Juan C JC Xu Bin B

Scientific reports 20160524

Production of membrane-associated cell surface receptors and their ligands is often a cumbersome, expensive, and time-consuming process that limits detailed structural and functional characterization of this important class of proteins. Here we report a rapid method for refolding inclusion-body-based, recombinant cell surface receptors and ligands in one day, a speed equivalent to that of soluble protein production. This method efficiently couples modular on-column immobilized metal ion affinity ...[more]

PMID: 27215173

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