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On-virus construction of polyvalent glycan ligands for cell-surface receptors.

ABSTRACT: Glycans arrayed on the exterior of virus particles were used as substrates for glycosyltransferase reactions to build di- and trisaccharides from the virus surface. The resulting particles exhibited tight and specific associations with cognate receptors on beads and cells, in one example defeating in cis cell-surface interactions in a manner characteristic of polyvalent binding. Combined with the ability of viruses to provide structurally well-defined attachment points, the methodology provides a convenient and powerful way to prepare complex carbohydrate ligands for clustered receptors.

SUBMITTER: Kaltgrad E

PROVIDER: S-EPMC5469293 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature

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On-virus construction of polyvalent glycan ligands for cell-surface receptors.

Kaltgrad Eiton E O'Reilly Mary K MK Liao Liang L Han Shoufa S Paulson James C JC Finn M G MG

Journal of the American Chemical Society 20080315 14

Glycans arrayed on the exterior of virus particles were used as substrates for glycosyltransferase reactions to build di- and trisaccharides from the virus surface. The resulting particles exhibited tight and specific associations with cognate receptors on beads and cells, in one example defeating in cis cell-surface interactions in a manner characteristic of polyvalent binding. Combined with the ability of viruses to provide structurally well-defined attachment points, the methodology provides ...[more]

PMID: 18341338

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