Ontology highlight
ABSTRACT:
SUBMITTER: Cummings CF
PROVIDER: S-EPMC4878091 | biostudies-literature | 2016 May
REPOSITORIES: biostudies-literature
Cummings Christopher F CF Pedchenko Vadim V Brown Kyle L KL Colon Selene S Rafi Mohamed M Jones-Paris Celestial C Pokydeshava Elena E Liu Min M Pastor-Pareja Jose C JC Stothers Cody C Ero-Tolliver Isi A IA McCall A Scott AS Vanacore Roberto R Bhave Gautam G Santoro Samuel S Blackwell Timothy S TS Zent Roy R Pozzi Ambra A Hudson Billy G BG
The Journal of cell biology 20160501 4
Basement membranes are defining features of the cellular microenvironment; however, little is known regarding their assembly outside cells. We report that extracellular Cl(-) ions signal the assembly of collagen IV networks outside cells by triggering a conformational switch within collagen IV noncollagenous 1 (NC1) domains. Depletion of Cl(-) in cell culture perturbed collagen IV networks, disrupted matrix architecture, and repositioned basement membrane proteins. Phylogenetic evidence indicate ...[more]