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Influences of Probe's Morphology for Metal Ion Detection Based on Light-Addressable Potentiometric Sensors.

ABSTRACT: The sensing mechanism of binding Hg(2+) into thymine-thymine (T-T) mismatched base pairs was introduced into a light-addressable potentiometric sensor (LAPS) with anti-Hg(2+) aptamer as the sensing units. Three kinds of T-rich single-strand DNA (ssDNA) chains with different spacer lengths, from 0 to 12 -CH? groups, were designed to investigate surface charge and morphological effects on the LAPS' output. First, by comparing the responding of LAPS modified with three kinds of ssDNA, it was found that the best performance for Hg(2+) sensing was exhibited by the probe without -CH? groups. The detection limit of Hg(2+) ion was 1 ppt under the optimal condition. Second, the cooperative effects of surface charge and morphology on the output were observed by the controlled experiments. The two effects were the negative charge balanced by metal cations and the morphological changing caused by the formation of T-Hg(2+)-T structure. In conclusion, not only the influences of the aptamer probe's morphology and surface charge was investigated on the platform of LAPS, but also sensing Hg(2+) ions was achieved for the first time by the presented aptamer LAPS.

SUBMITTER: Shao C

PROVIDER: S-EPMC4883392 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature

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Influences of Probe's Morphology for Metal Ion Detection Based on Light-Addressable Potentiometric Sensors.

Shao Chen C Zhou Shuang S Yin Xuebo X Gu Yajun Y Jia Yunfang Y

Sensors (Basel, Switzerland) 20160514 5

The sensing mechanism of binding Hg(2+) into thymine-thymine (T-T) mismatched base pairs was introduced into a light-addressable potentiometric sensor (LAPS) with anti-Hg(2+) aptamer as the sensing units. Three kinds of T-rich single-strand DNA (ssDNA) chains with different spacer lengths, from 0 to 12 -CH₂ groups, were designed to investigate surface charge and morphological effects on the LAPS' output. First, by comparing the responding of LAPS modified with three kinds of ssDNA, it was found ...[more]

PMID: 27187412

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Project description:DNA methylation (DNAm) sensors are an emerging branch in the discipline of sensors. It is believed to be able to promote the next generation of epigenetics-based diagnostic technology. Differing from the traditional biochemical sensors that aimed at individual molecules, the challenge in DNAm sensors is how to determine the amount of 5-methylcytosine (5mC) in a continuous nucleotide sequence. Here, we report a comparative study about meso-tetra(4-carboxyphenyl)porphine (TCPP)-based DNAm sensing interfaces on a light-addressable potentiometric sensor (LAPS), depending on TCPP's postures that are flat in the π-conjugated TCPP layer on reduced-graphene-oxide-decorated LAPS (#1) and stand-up in the covalently anchored TCPP on glutaraldehyde (GA)-treated LAPS (#2), along with the blank one (only GA-treated LAPS, #3). These DNAm sensing interfaces are also distinct from the traditional biosensing interface on LAPS, that is: it is not functionalized by the sensing indicator (5mC antibody, in this case) but by the target nucleotide sequence. The surface characterization techniques such as Raman spectra, scanning electron microscopy, and X-ray photoelectron spectroscopy are conducted to prove the decorations, as well as the anchored nucleotides. It is found that, though all of them can detect as low as one 5mC in the target sequence, the enhanced DNAm sensitivity is obtained by #2, which is evidenced by the higher output-voltage changing ratio for the 5mC site of #2 than those of #1 and #3. Furthermore, the underlying causes for the improved sensitivity in #2 are proposed, according to the conformational and electronic properties of TCPP molecules. Conclusively, TCPP's synergetic function, including the molecular configuration and the activate (carboxyl) groups on its peripheral substituents, to improve the DNAm sensing interface on LAPS is investigated and demonstrated. This can shed light on a new approach for DNA methylation detection, with the merits of low cost, independence on bisulfite conversion, and polymerase chain reaction.

Dataset Information

Influences of Probe's Morphology for Metal Ion Detection Based on Light-Addressable Potentiometric Sensors.

Publications

Influences of Probe's Morphology for Metal Ion Detection Based on Light-Addressable Potentiometric Sensors.

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