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Chemical methods for the simultaneous quantitation of metabolites and proteins from single cells.

ABSTRACT: We describe chemical approaches for integrated metabolic and proteomic assays from single cells. Quantitative assays for intracellular metabolites, including glucose uptake and three other species, are designed as surface-competitive binding assays with fluorescence readouts. This enables integration into a microarray format with functional protein immunoassays, all of which are incorporated into the microchambers of a single-cell barcode chip (SCBC). By using the SCBC, we interrogate the response of human-derived glioblastoma cancer cells to epidermal growth factor receptor inhibition. We report, for the first time, on both the intercellular metabolic heterogeneity as well as the baseline and drug-induced changes in the metabolite-phosphoprotein correlation network.

SUBMITTER: Xue M

PROVIDER: S-EPMC4883658 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature

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Chemical methods for the simultaneous quantitation of metabolites and proteins from single cells.

Xue Min M Wei Wei W Su Yapeng Y Kim Jungwoo J Shin Young Shik YS Mai Wilson X WX Nathanson David A DA Heath James R JR

Journal of the American Chemical Society 20150319 12

We describe chemical approaches for integrated metabolic and proteomic assays from single cells. Quantitative assays for intracellular metabolites, including glucose uptake and three other species, are designed as surface-competitive binding assays with fluorescence readouts. This enables integration into a microarray format with functional protein immunoassays, all of which are incorporated into the microchambers of a single-cell barcode chip (SCBC). By using the SCBC, we interrogate the respon ...[more]

PMID: 25789560

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