Ontology highlight
ABSTRACT:
SUBMITTER: Blount BA
PROVIDER: S-EPMC4886510 | biostudies-literature | 2016 May
REPOSITORIES: biostudies-literature
Blount Benjamin A BA Driessen Maureen R M MR Ellis Tom T
Scientific reports 20160531
Existing yeast genomic DNA extraction methods are not ideally suited to extensive screening of colonies by PCR, due to being too lengthy, too laborious or yielding poor quality DNA and inconsistent results. We developed the GC prep method as a solution to this problem. Yeast cells from colonies or liquid cultures are lysed by vortex mixing with glass beads and then boiled in the presence of a metal chelating resin. In around 12 minutes, multiple samples can be processed to extract high yields of ...[more]