Ontology highlight
ABSTRACT:
SUBMITTER: Laidlaw SM
PROVIDER: S-EPMC4886819 | biostudies-literature | 2014 May
REPOSITORIES: biostudies-literature
Bio-protocol 20140501 10
The construction of deletion-knockout poxviruses is a useful approach to determining the function of specific virus genes. This protocol is an adaptation of the transient dominant knockout selection protocol published by Falkner and Moss (1990) for use with vaccinia virus. The protocol makes use of the dominant selectable marker <i>Escherichia coli</i> guanine phosphoribosyltransferase (gpt) gene (Mulligan and Berg, 1981), under the control of an early/late poxvirus promoter. The deletion viruse ...[more]