Project description:This project describes the protein composition of the Cafeteria roenbergensis virus (CroV, strain BV-PW1: TaxID 693272) particle, a giant marine DNA virus that infects the heterotrophic nanoflagellate microeukaryote C. roenbergensis. CroV is a member of the Nucleo-Cytoplasmic Large DNA Virus clade and related to Acanthamoeba polyphaga mimivirus. CroV possesses a DNA genome of ~730 kilobase pairs that encodes 544 predicted proteins. We analyzed the protein composition of purified CroV particles by liquid chromatography - tandem mass spectrometry (LC-MS/MS) and identified 141 virion-associated CroV proteins. Predicted functions could be assigned to 37% of these proteins, which include structural proteins as well as enzymes for transcription, DNA repair, redox reactions and protein modification. Homologs of 36 CroV virion proteins have previously been found in the virion of Acanthamoeba polyphaga mimivirus. This study shows that giant DNA virus particles contain more than one hundred viral proteins that include specific enzymatic functions.
Project description:WT and GSDMD KO mice were infected with 50 TCID50 influenza virus strain PR8. 7 days post infection, RNA was extracted from lung tissue using TRIzol. RNA library preparation and sequencing was performed by Genewiz.
Project description:To obtain the site-by-site methylation landscape of the infectious spleen and kidney necrosis virus (ISKNV) genome, whole-genome bisulfite sequencing (WGBS) was performed on an ISKNV strain from 3 duplicate samples.
Project description:Analysis of gene expression in lungs of C57BL/6J mice that develop chronic airway disease phenotypes after a single Sendai virus infection, compared with mice treated with UV-inactivated virus. Experiment Overall Design: Whole lung RNA was analyzed from 3 mice per condition per time point for days 21 and 49 post infection, Sendai virus versus UV-inactivated virus, C57BL/6J mouse strain.
Project description:Ten cattle have been challenged with two Lumpy Skin Disease Virus (LSDV). They were sampled for whole blood immediately before (pre) and three and seven days after (post) infection challenge with two virus strains (H vs. O). The whole RNA-sequencing was done, and 150bp paired reads were assembled as the transcriptome. It was then computationally analyzed to find the differentially expressed genes (DGE) that enrich the gene ontology (GO) terms and KEGG pathways. Depending on the challenged LSDV strain, they influence the host response differently.
