Dataset Information

Bacterial Community Diversity Harboured by Interacting Species.

ABSTRACT: All animals are infected by microbial partners that can be passengers or residents and influence many biological traits of their hosts. Even if important factors that structure the composition and abundance of microbial communities within and among host individuals have been recently described, such as diet, developmental stage or phylogeny, few studies have conducted cross-taxonomic comparisons, especially on host species related by trophic relationships. Here, we describe and compare the microbial communities associated with the cabbage root fly Delia radicum and its three major parasitoids: the two staphylinid beetles Aleochara bilineata and A. bipustulata and the hymenopteran parasitoid Trybliographa rapae. For each species, two populations from Western France were sampled and microbial communities were described through culture independent methods (454 pyrosequencing). Each sample harbored at least 59 to 261 different bacterial phylotypes but was strongly dominated by one or two. Microbial communities differed markedly in terms of composition and abundance, being mainly influenced by phylogenetic proximity but also geography to a minor extent. Surprisingly, despite their strong trophic interaction, parasitoids shared a very low proportion of microbial partners with their insect host. Three vertically transmitted symbionts from the genus Wolbachia, Rickettsia, and Spiroplasma were found in this study. Among them, Wolbachia and Spiroplasma were found in both the cabbage fly and at least one of its parasitoids, which could result from horizontal transfers through trophic interactions. Phylogenetic analysis showed that this hypothesis may explain some but not all cases. More work is needed to understand the dynamics of symbiotic associations within trophic network and the effect of these bacterial communities on the fitness of their hosts.

SUBMITTER: Bili M

PROVIDER: S-EPMC4892616 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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Bacterial Community Diversity Harboured by Interacting Species.

Bili Mikaël M Cortesero Anne Marie AM Mougel Christophe C Gauthier Jean Pierre JP Ermel Gwennola G Simon Jean Christophe JC Outreman Yannick Y Terrat Sébastien S Mahéo Frédérique F Poinsot Denis D

PloS one 20160603 6

All animals are infected by microbial partners that can be passengers or residents and influence many biological traits of their hosts. Even if important factors that structure the composition and abundance of microbial communities within and among host individuals have been recently described, such as diet, developmental stage or phylogeny, few studies have conducted cross-taxonomic comparisons, especially on host species related by trophic relationships. Here, we describe and compare the micro ...[more]

PMID: 27258532

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Project description:IntroductionPlant species composition in forest ecosystems can alter soil greenhouse gas (GHG) budgets by affecting soil properties and microbial communities. However, little attention has been paid to the forest types characterized by understory vegetation, especially in boreal forests where understory species contribute significantly to carbon and nitrogen cycling.MethodIn the present study, soil GHG fluxes, soil properties and bacterial community, and soil environmental conditions were investigated among three types of larch forest [Rhododendron simsii-Larix gmelinii forest (RL), Ledum palustre-Larix gmelinii forest (LL), and Sphagnum-Bryum-Ledum palustre-Larix gmelinii forest (SLL)] in the typical boreal region of northeast China to explore whether the forest types characterized by different understory species can affect soil GHG fluxes.ResultsThe results showed that differences in understory species significantly affected soil GHG fluxes, properties, and bacterial composition among types of larch forest. Soil CO2 and N2O fluxes were significantly higher in LL (347.12 mg m-2 h-1 and 20.71 μg m-2 h-1) and RL (335.54 mg m-2 h-1 and 20.73 μg m-2 h-1) than that in SLL (295.58 mg m-2 h-1 and 17.65 μg m-2 h-1), while lower soil CH4 uptake (-21.07 μg m-2 h-1) were found in SLL than in RL (-35.21 μg m-2 h-1) and LL (-35.85 μg m-2 h-1). No significant differences between LL and RL were found in soil CO2, CH4, and N2O fluxes. Soil bacterial composition was mainly dominated by Proteobacteria, Actinobacteria, Acidobacteria, and Chloroflexi among the three types of larch forest, while their abundances differed significantly. Soil environmental variables, soil properties, bacterial composition, and their interactions significantly affected the variations in GHG fluxes with understory species. Specifically, structural equation modeling suggested that soil bacterial composition and temperature had direct close links with variations in soil GHG fluxes among types of larch forest. Moreover, soil NO3 --N and NH4 + - N content also affected soil CO2, CH4, and N2O fluxes indirectly, via their effects on soil bacterial composition.DiscussionOur study highlights the importance of understory species in regulating soil GHG fluxes in boreal forests, which furthers our understanding of the role of boreal forests in sustainable development and climate change mitigation.

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Bacterial Community Diversity Harboured by Interacting Species.

Publications

Bacterial Community Diversity Harboured by Interacting Species.

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