O-glycosylation sites identified from mucin core-1 type glycopeptides from human serum.
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ABSTRACT:
SUBMITTER: Darula Z
PROVIDER: S-EPMC4892982 | biostudies-literature | 2016 Jun
REPOSITORIES: biostudies-literature
Publications
O-glycosylation sites identified from mucin core-1 type glycopeptides from human serum.
Darula Zsuzsanna Z Sarnyai Farkas F Medzihradszky Katalin F KF
Glycoconjugate journal 20160105 3
In this work O-linked glycopeptides bearing mucin core-1 type structures were enriched from human serum. Since about 70 % of the O-glycans in human serum bind to the plant lectin Jacalin, we tested a previously successful protocol that combined Jacalin affinity enrichment on the protein- and peptide-level with ERLIC chromatography as a further enrichment step in between, to eliminate the high background of unmodified peptides. In parallel, we developed a simpler and significantly faster new work ...[more]
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