Ontology highlight
ABSTRACT:
SUBMITTER: Garcia-Nafria J
PROVIDER: S-EPMC4893743 | biostudies-literature | 2016 Jun
REPOSITORIES: biostudies-literature
García-Nafría Javier J Watson Jake F JF Greger Ingo H IH
Scientific reports 20160606
In vivo homologous recombination holds the potential for optimal molecular cloning, however, current strategies require specialised bacterial strains or laborious protocols. Here, we exploit a recA-independent recombination pathway, present in widespread laboratory E.coli strains, to develop IVA (In Vivo Assembly) cloning. This system eliminates the need for enzymatic assembly and reduces all molecular cloning procedures to a single-tube, single-step PCR, performed in <2 hours from setup to tran ...[more]