Ontology highlight
ABSTRACT:
SUBMITTER: Weber E
PROVIDER: S-EPMC3041749 | biostudies-literature | 2011 Feb
REPOSITORIES: biostudies-literature
PloS one 20110218 2
The field of synthetic biology promises to revolutionize biotechnology through the design of organisms with novel phenotypes useful for medicine, agriculture and industry. However, a limiting factor is the ability of current methods to assemble complex DNA molecules encoding multiple genetic elements in various predefined arrangements. We present here a hierarchical modular cloning system that allows the creation at will and with high efficiency of any eukaryotic multigene construct, starting fr ...[more]