Ontology highlight
ABSTRACT:
SUBMITTER: Frauenfeld J
PROVIDER: S-EPMC4894539 | biostudies-literature | 2016 Apr
REPOSITORIES: biostudies-literature
Nature methods 20160307 4
A limiting factor in membrane protein research is the ability to solubilize and stabilize such proteins. Detergents are used most often for solubilizing membrane proteins, but they are associated with protein instability and poor compatibility with structural and biophysical studies. Here we present a saposin-lipoprotein nanoparticle system, Salipro, which allows for the reconstitution of membrane proteins in a lipid environment that is stabilized by a scaffold of saposin proteins. We demonstrat ...[more]