Ontology highlight
ABSTRACT:
SUBMITTER: Meier D
PROVIDER: S-EPMC4894637 | biostudies-literature | 2016 Jun
REPOSITORIES: biostudies-literature
Meier Doreen D Kruse Janis J Buttlar Jann J Friedrich Michael M Zenk Fides F Boesler Benjamin B Förstner Konrad U KU Hammann Christian C Nellen Wolfgang W
PLoS genetics 20160606 6
We identified the dsRNA binding protein RbdB as an essential component in miRNA processing in Dictyostelium discoideum. RbdB is a nuclear protein that accumulates, together with Dicer B, in nucleolar foci reminiscent of plant dicing bodies. Disruption of rbdB results in loss of miRNAs and accumulation of primary miRNAs. The phenotype can be rescued by ectopic expression of RbdB thus allowing for a detailed analysis of domain function. The lack of cytoplasmic dsRBD proteins involved in miRNA proc ...[more]