Ontology highlight
ABSTRACT:
SUBMITTER: Fiaschi L
PROVIDER: S-EPMC4895005 | biostudies-literature | 2016 Jun
REPOSITORIES: biostudies-literature
Fiaschi Luigi L Di Palo Benedetta B Scarselli Maria M Pozzi Clarissa C Tomaszewski Kelly K Galletti Bruno B Nardi-Dei Vincenzo V Arcidiacono Letizia L Mishra Ravi P N RP Mori Elena E Pallaoro Michele M Falugi Fabiana F Torre Antonina A Fontana Maria Rita MR Soriani Marco M Bubeck Wardenburg Juliane J Grandi Guido G Rappuoli Rino R Ferlenghi Ilaria I Bagnoli Fabio F
Clinical and vaccine immunology : CVI 20160606 6
Staphylococcus aureus alpha-hemolysin (Hla) assembles into heptameric pores on the host cell membrane, causing lysis, apoptosis, and junction disruption. Herein, we present the design of a newly engineered S. aureus alpha-toxin, HlaPSGS, which lacks the predicted membrane-spanning stem domain. This protein is able to form heptamers in aqueous solution in the absence of lipophilic substrata, and its structure, obtained by transmission electron microscopy and single-particle reconstruction analysi ...[more]