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Estrogen receptor profiling and activity in cardiac myocytes.

ABSTRACT: Estrogen signaling appears critical in the heart. However a mechanistic understanding of the role of estrogen in the cardiac myocyte is lacking. Moreover, there are multiple cell types in the heart and multiple estrogen receptor (ER) isoforms. Therefore, we studied expression, localization, transcriptional and signaling activity of ERs in isolated cardiac myocytes. We found only ER? RNA (but no ER? RNA) in cardiac myocytes using two independent methods. The vast majority of full-length ER? protein (ER?66) localizes to cardiac myocyte nuclei where it is competent to activate transcription. Alternate isoforms of ER? encoded by the same genomic locus (ER?46 and ER?36) have differential transcriptional activity in cardiac myocytes but also primarily localize to nuclei. In contrast to other reports, no ER? isoform is competent to activate MAPK or PI3K signaling in cardiac myocytes. Together these data support a role for ER? at the level of transcription in cardiac myocytes.

SUBMITTER: Pugach EK

PROVIDER: S-EPMC4899180 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature

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Estrogen receptor profiling and activity in cardiac myocytes.

Pugach Emily K EK Blenck Christa L CL Dragavon Joseph M JM Langer Stephen J SJ Leinwand Leslie A LA

Molecular and cellular endocrinology 20160507

Estrogen signaling appears critical in the heart. However a mechanistic understanding of the role of estrogen in the cardiac myocyte is lacking. Moreover, there are multiple cell types in the heart and multiple estrogen receptor (ER) isoforms. Therefore, we studied expression, localization, transcriptional and signaling activity of ERs in isolated cardiac myocytes. We found only ERα RNA (but no ERβ RNA) in cardiac myocytes using two independent methods. The vast majority of full-length ERα prote ...[more]

PMID: 27164442

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