Ontology highlight
ABSTRACT:
SUBMITTER: Andersen TG
PROVIDER: S-EPMC4901268 | biostudies-literature | 2016 Jun
REPOSITORIES: biostudies-literature
Andersen Tonni Grube TG Nintemann Sebastian J SJ Marek Magdalena M Halkier Barbara A BA Schulz Alexander A Burow Meike M
Scientific reports 20160610
When investigating interactions between two proteins with complementary reporter tags in yeast two-hybrid or split GFP assays, it remains troublesome to discriminate true- from false-negative results and challenging to compare the level of interaction across experiments. This leads to decreased sensitivity and renders analysis of weak or transient interactions difficult to perform. In this work, we describe the development of reporters that can be chemically induced to dimerize independently of ...[more]