Kinetics of Protein Complex Dissociation Studied by Hydrogen/Deuterium Exchange and Mass Spectrometry.

ABSTRACT: The growing importance of protein aggregation diseases requires the development of new methods to elucidate the molecular features that are responsible for the incipient protein-protein interactions. Kinetic information from protein-protein association/dissociation reactions is particularly valuable for revealing mechanistic insight, but robust tools that can provide this information are somewhat lacking. In this work, we describe a hydrogen/deuterium exchange (HDX)-based method that provides rate constant information for protein oligomer dissociation, using the well-studied β-lactoglobulin (βLG) dimer as a model system to validate our approach. By measuring the rate of exchange at different regions of the protein using top-down tandem mass spectrometry and fitting the resulting data to an

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC4902019 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature